FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35665009
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG640570.1796074129688289No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494020.13851671816485453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT40900.048.94791420
CTCGCGT60250.048.0417826
GTCTACG57000.046.1129464
TAGCGCG36200.044.9655721
TCGCGTC64600.044.75340727
GCGTTAC45800.043.7113116
CGATCCA66300.043.4455575
TGCGTTA46200.043.1815315
GTTACCG46700.043.16869418
TCTACGG61500.042.795865
TACGGCC62250.042.2791827
TAACCCC167100.042.1071051
TTACCGT47850.041.91129719
CGTTACC49100.041.05850217
AAACGCG25150.039.80741
GCGATCC73700.039.320654
GAGTCGC73350.039.03161236
ACCGTTT51650.038.69254321
AGAGTCG77400.037.17049835
TTGCGAC20550.037.1347431
Produced by FastQC (version 0.11.5)