FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26048273
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG512310.19667714631215666No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396230.15211373130187938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC137050.038.8704721
TACCGTT25700.034.72378520
TTACCGT25600.034.7225919
ACCGTTT29600.030.38556321
TTGGGGG212250.030.35921
GTTACCG29550.029.84430118
TTGCGAG26300.029.1455731
TGCGTTA31650.028.52792415
CGTTACC32100.028.0187117
TTGCGAC17600.026.2509771
TTGGGGC160000.025.660331
TAGCGCG10950.025.5717281
CCGTTTG35500.025.53292722
GCGTTAC35350.025.3438316
TAGGGGG64050.024.6457231
TTACCCC82750.024.448041
CTGCGTT38250.023.78844614
TCGCACA41650.023.6983621
TTGCATC73550.023.5086941
TTACGCC17150.023.2661721
Produced by FastQC (version 0.11.5)