FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27633915
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440000.15922463393261505No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG321560.1163642574712993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG642200.034.83561369
ATCGCAC749100.031.0828420
AATCGCA760050.030.73443219
AAATCGC764400.030.70271518
TCGCACT767000.030.36648821
ACCACGA725350.030.29794954
ACGAACT747850.030.06541457
CGCACTG796000.029.20875222
CACGAAC758650.029.07742756
CGAACTG774300.029.03031258
CCACGAA761500.028.91370255
TCCTATC794550.028.80857544
GACTCTT799050.028.61923636
GGACTCT814950.028.10758635
TTCCTAT815850.028.03061943
CCTATCT826000.027.68274945
TGGACTC832750.027.62264834
CAAATCG850850.027.59945117
CCTGGAC838600.027.37225532
GAACTGC833650.027.1705759
Produced by FastQC (version 0.11.5)