FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35656826
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG676660.18977011582578887No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560200.15710876789762498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT57850.050.74967620
TTACCGT58000.050.7363919
GTTACCG64050.046.1590818
CGTTACC65450.045.17191317
ACCGTTT65200.045.08233321
GTCTACG55800.044.7034454
TGCGTTA66450.044.3368115
CGTTTGA69800.042.1614823
TAACCCC200950.042.1056181
GCGTTAC72600.040.86594816
CCGTTTG74300.040.02564222
CTGCGTT74100.039.94609514
TGTACCG81950.035.82522234
TCTACGG70900.035.7179645
TAGCGCG25750.035.1100651
TTGCGAC22600.032.522111
TCTCGTC74150.032.24153536
TTGGGGG305750.031.0027161
CCCGATC77700.030.94880131
TACGGCC83500.030.2012677
Produced by FastQC (version 0.11.5)