FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35656826
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG694870.19487713236169701No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG507420.1423065530285842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCCG73700.035.6702162
GCCGATC104750.033.06737569
CGCGAAC39400.032.3995935
GGCCGAT113650.030.68982768
TACGAAC29250.030.0779615
GCGAACT57800.029.8443986
TGCCGAC92900.028.63242564
CGATTAC61400.027.30676810
TTGCTCG886700.026.57054369
CCGCGAA45000.026.4524334
TTATCGA64850.026.2807436
CGACCTC100850.026.20391767
ATCGCAC952200.025.80969620
CGTTATA22400.025.4138225
AATCGCA968950.025.3956819
TTGCCGA104800.025.34833763
TCGCACT971900.025.3078421
AAATCGC982750.025.06009518
AGGTTAC109150.025.03180928
ACCACGA919950.024.99169354
Produced by FastQC (version 0.11.5)