FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35232108
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG729260.20698733098797267No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG524440.14885285887520555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG193750.061.6548584
TCTACGG200700.059.8163725
TACGGCC208950.057.3355067
CTACGGC215100.055.8608176
CGGCCAT220200.054.565349
GCCATAC227300.051.8601311
CGTTCGA30650.051.84189236
TACCGTT43350.051.51026520
TAGCGCG51500.050.1671261
GGTCTAC113850.049.8643153
ACGGCCA243100.049.6554268
TTGCGAC26950.048.323041
GGCCATA250250.048.15306510
GCGTTAC47500.047.15699416
GAACGCG246800.047.1243724
AAACGCG31300.046.9758951
TGCGTTA47650.046.7148115
CGTTACC48900.045.80676317
AACGCGC255300.045.43221325
TATCGCG10250.045.425311
Produced by FastQC (version 0.11.5)