FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27858779
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG573380.20581662965200304No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444700.1596265220381697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC144600.045.1010931
TACCGTT23450.038.05932620
GCGTAAC26300.037.3953
TTACCGT25050.036.18713819
TTGGGGG217900.032.1945951
CGCGTAA5250.032.0000882
TGCGTTA29100.031.75186515
TCGCACA50650.031.4466551
GTTACCG29700.031.11069318
ACCGTTT29200.030.68457621
GCGTTAC30000.030.33281716
TAGGGGG72100.029.8109361
CGTTACC31100.029.71016117
TAACCCG22000.027.3683261
TTGCGAG29950.027.3502661
TGGGTAG91850.026.907171
AACCCCT167650.026.8267962
TTGGGAC141500.026.6441461
TGGGAAG472150.026.4982411
TTGGGGC167850.026.1945421
Produced by FastQC (version 0.11.5)