FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27858779
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564570.20265425128646164No Hit
TCCTTGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC298900.10729113433147949No Hit
GCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCTGGGT290440.10425438961269623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC31800.039.3852655
GCGAACT48600.033.402396
ATTGCCG47150.032.6711862
CCGCGAA36450.032.0545274
TGCTCGC1217700.030.73905670
TACGAAC25050.030.026775
GACTACG75100.029.41211748
ACTACGC77200.028.79430249
ATCGCAC1387500.028.44293820
TACGCTG79300.028.25321451
TCGCACT1401750.028.17477621
AAATCGC1410450.028.15187518
AATCGCA1404950.028.12997219
CTACGCT79550.028.12061750
GTAAAGC149350.027.75409354
ACCACGA1290100.027.68487254
GGACTCT1400750.027.51168335
GACTCTT1392900.027.50883136
CGCACTG1436850.027.40648322
TTGCTCG1385300.027.29644869
Produced by FastQC (version 0.11.5)