FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27072013
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG537870.19868119891934152No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394610.14576308012263442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC143750.045.728371
TACCGTT23950.031.70928220
TTGGGGG235400.029.738671
TTACCGT25900.029.72689219
TTGCGAG29050.029.3996161
ACCGTTT26900.028.49244321
GTTACCG29250.026.32227318
TTACCCC85750.025.7160591
TAGGGGG71800.025.6911871
AACCCCT162600.025.6557042
TAGCGCG16150.025.574521
TTGGGCC132350.025.468321
TTGGGGC181950.025.2202071
TTGCGAC23650.025.1602971
ATACGTC44750.024.87032938
CGTTACC31250.024.7497317
TTGGGAC148750.024.542871
TCGTCGG37400.024.42357323
CTATCCG44850.024.26877247
TGGGGGG405850.024.2606351
Produced by FastQC (version 0.11.5)