FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27072013
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494930.18281979991661498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGC734300.032.2643870
ATCGCAC835100.030.43016220
AATCGCA845700.030.05977419
AAATCGC852700.030.00998318
TCGCACT850450.029.90661221
ACCACGA760950.029.5161454
ACGAACT812900.028.88706857
TTGCTCG837550.028.59925569
CGCACTG891950.028.47281522
CACGAAC799450.028.32590156
CCACGAA800100.028.1765555
TCCTATC873550.028.05419244
GACTCTT863400.027.8769536
CGAACTG848700.027.83640358
GGACTCT879200.027.65058935
CAAATCG928500.027.43530317
TGGACTC893900.027.42833934
CCTGGAC899150.027.40152532
CACCACG843350.027.31218153
TTCCTAT901650.027.18376443
Produced by FastQC (version 0.11.5)