FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34047114
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG479050.14070208711375654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG87800.053.9764825
TACCGTT47550.052.5551620
TACGGCC93350.050.577387
TTACCGT51450.049.11538719
GTCTACG100300.047.4240154
TGCGTTA52800.047.39495515
GTTACCG55250.045.54720718
GCGTTAC54950.045.54069516
CTACGGC104700.045.0633666
CGTTACC56050.045.0219317
AAACGCG34400.044.9788171
ACCGTTT56000.044.50015621
AACCCCC345300.043.6679422
CTCGCGT45200.043.5965726
CGGCCAT109550.043.1300669
GCCATAC108700.042.79110711
TGTACCG58200.042.3979334
TAGCGCG34450.041.8651051
ATACGCG9950.041.5148161
AACCCGC70600.041.049632
Produced by FastQC (version 0.11.5)