FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34047114
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA1007150.29581068163369145No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG737800.2166997179261655No Hit
GGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCG683890.20086577675864098No Hit
ACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGC647520.19018352040058373No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG640710.18818335087079627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG609300.1789578993391334No Hit
GCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGG605750.1779152265299197No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCC508290.14929018653387185No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA457840.13447248421701763No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG456830.13417583646003006No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA443190.1301696231874455No Hit
TTGACATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGC435730.12797854173484424No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA399010.11719348664911804No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA386160.11341930479041484No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT385090.11310503439439831No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA383090.11251761309343282No Hit
TTTGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC371670.10916343746491994No Hit
TGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCCGAC369080.10840272688016964No Hit
GCTGCGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG363570.10678438119600975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACG52400.042.3889774
TGTACGA57550.041.21125
TGCGTAC101150.037.6215323
GTTACGT27600.037.0039331
GCGTACG105050.036.261214
CTGCGTA102100.036.0375442
CGGACTA26100.033.640635
GCCTACG31750.033.378981
ACGAGTG27950.033.286941
GCTTACG28300.031.7629491
GCTTCGT50600.031.4510381
CGGAACG100250.030.97456746
ACCGTCC242350.030.64963510
GTCGGTA22500.030.6262764
CGCCCTA165100.030.53954761
AACGGCG104300.029.57221249
GTTCGTA28550.029.5246263
TTATCGG35900.028.9407757
GTTATCG36950.028.8753856
CTTACGT30100.028.3523122
Produced by FastQC (version 0.11.5)