FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33632267
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGG677080.20131857302393563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG624010.1855390836425032No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATC369780.10994798536774224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC105950.033.4089569
CGATTAC87300.032.56289310
TTATCGA89250.031.9299266
TATCGAT91350.031.271077
GGCCGAT114950.031.12310868
ATTGCCG43150.030.22265662
ATCGATT96800.029.510028
TTGCTCG488800.028.22517269
TTTATCG104750.027.0741525
TCGATTA106700.026.7394439
ATCGCAC541000.026.29062320
AATCGCA556500.025.63297719
CGCGAAC20250.025.5566165
TCGCACT560250.025.38086321
CGGTAGG76600.025.17714960
AAATCGC571500.025.03866418
ACCACGA530200.025.00642454
CTCGGTA78350.024.83456258
ACGAACT561600.024.56032257
TTGTCGC78300.024.27744968
Produced by FastQC (version 0.11.5)