FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23862439
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG345340.1447211661808753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG375050.030.7432769
GCCGATC41900.029.16634669
ATCGCAC434050.027.92445820
AATCGCA438000.027.6954419
AAATCGC442500.027.60790618
TCGCACT444100.027.24712821
ACCACGA412600.026.94446254
ACGAACT431250.026.61279957
CGCACTG460550.026.28916422
CACGAAC423350.026.26869856
CCACGAA427950.026.1154955
GGCCGAT47700.025.76476768
CGAACTG448150.025.75566558
TCCTATC472350.025.03927444
ATTGCCG25600.024.8107962
CAAATCG501050.024.4565417
CACCACG472950.024.00180253
GACTCTT493850.023.84708436
GGACTCT499100.023.81727835
CCTATCT497600.023.6298745
Produced by FastQC (version 0.11.5)