FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30242945
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415130.1372650712422352No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA306580.10137240272070065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC19800.030.7563045
GCCGATC70600.029.2564669
GGCCGAT76850.026.9669768
TGCTCGC582650.026.73305170
CGGATAG58350.025.8533435
ATCGCAC643800.025.36909320
ACCACGA586900.025.17897854
TCGGATA59700.025.15150634
CCGATCC82050.025.04686570
GCGAACT35100.025.0285326
AATCGCA658850.024.800319
TCGCACT659950.024.77993221
AAATCGC667250.024.68239418
ACGAACT630450.024.5017657
TTGCTCG646450.024.38605169
CGATTAC31400.023.96766910
ACGTATC65750.023.79192245
GACGTAT65500.023.66964144
CGCACTG691500.023.6175222
GGACGTA66000.023.54416843
Produced by FastQC (version 0.11.5)