FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29291718
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG636570.21732081402668155No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG399890.13651981764948032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC125950.045.1982541
GCGTAAC28800.035.854553
CTATCCG54100.035.3867247
TATCCGA54450.035.28792648
TACGTCC58150.033.4031639
GATACGT58950.033.30629737
ATACGTC58950.033.06853538
TGATACG65100.029.72955736
GTAGTAT70250.027.6486477
GTGTAGT70150.027.4941865
TAGCGGA20900.025.6297531
AACCCCT166800.025.3085612
AACCCCC153000.025.3034612
TAGTATC78000.025.260368
TCTTATC81100.023.82036618
TTGCGAG31700.023.6349011
TTGCACC101700.023.2371081
CTCTATC85350.023.12718845
TTGGGGG219500.023.0639151
TCTATCC83800.023.01222246
Produced by FastQC (version 0.11.5)