FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31091608
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG809700.26042397035238574No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG469910.15113724578027615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC187750.044.883461
GCGTAAC28500.032.9157683
AACCCCC180150.031.4575772
TTACCCC80800.030.1569981
TAGCGCG11200.028.7580911
GTCTACG34650.028.689824
AACCCCT211600.027.9234752
TAGCACG20650.027.8044321
TTGCGAC20400.026.6006221
CTATCCG39000.026.56328847
TGGGGGG421200.026.1160831
TTGGGGG221450.026.069651
CGTCGAT24200.025.88725935
ATACGTC41000.024.84006138
CGCGTAA4950.024.750022
TACGTCC41350.024.71448539
TATCCGA40800.024.70516648
TTGCAAC106000.024.7050631
AACCCCA338650.024.6828862
AACGCGC38600.024.66217625
Produced by FastQC (version 0.11.5)