FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31091608
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790190.2541489652127352No Hit
TCCTGGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC434540.13976118571931048No Hit
TCCTTGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC403040.12962983451997723No Hit
TCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTCCTATC392110.12611441646890698No Hit
GCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCTGGGT324580.10439472927871726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC32750.043.4825335
GCGAACT46850.039.1362576
TACGAAC23750.036.5358965
CCGCGAA37700.035.8234334
TGCTCGC1246450.033.22231770
ATCGCAC1369450.031.1412620
TCGCACT1381850.030.8491521
AATCGCA1385200.030.83533719
AAATCGC1394800.030.75034718
CGCACTG1405850.030.24776522
ACCACGA1327700.030.23089254
ATTGCCG42500.030.1464662
GGACTCT1402900.029.8931235
GACTCTT1409000.029.72185736
TTGCTCG1411700.029.48898569
TCCTATC1421200.029.4711244
TGGACTC1431650.029.3980734
ACGAACT1417050.029.31437557
CCACGAA1374250.029.27497555
CCTGGAC1442700.029.14832
Produced by FastQC (version 0.11.5)