FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28729486
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG643410.22395458101826118No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG594080.2067840684654087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT27950.038.80006820
TAACCCC165450.038.5503581
TAGCGCG15300.037.5025631
TTACCGT29200.036.65931719
TTGGGGG263450.034.8477021
CTATCCG79500.034.04715747
GTTACCG32000.033.67026518
TTGCGAG35500.033.4102631
TATCCGA81300.033.2507448
ATACGTC84050.032.45168338
ACCGTTT33750.032.4433321
TACGTCC87400.031.16999239
CGTTACC35300.030.5227817
GATACGT89650.030.50136837
TGCGTTA36950.029.44396615
TTACCCG24750.029.403341
TGATACG94600.029.12656836
GCGTTAC37750.028.72716716
TTGGGGC187500.028.4375511
TTGGGAC164100.028.1005841
Produced by FastQC (version 0.11.5)