FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28729486
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540800.18823866184031277No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG378330.13168700616502502No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA308670.10744014007072733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC40300.038.992665
GCCGATC85850.035.15175669
CCGCGAA39850.034.6013954
GCGAACT70800.033.912086
GGCCGAT90400.033.61334268
TACGAAC42750.032.9102755
CCGATCC93800.031.91349670
ATTGCCG45350.030.33176662
CGGATAG73200.027.11089335
GACTACG45900.026.91163348
TCGGATA74600.026.5088334
GACGTAT77350.025.74422844
ACTACGC47550.025.60907649
CGATTAC28350.025.5586310
ACCGCGA26100.025.3417823
ATCGCAC927350.024.98318520
ACGTATC80300.024.71060645
TCGCACT935750.024.69528421
AATCGCA945200.024.61937319
AAATCGC948350.024.5898718
Produced by FastQC (version 0.11.5)