FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30521987
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG622920.20408894086744747No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG498250.16324297628460427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322380.10562221915630854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT45700.047.40585719
TACCGTT46800.046.2172820
TAACCCC170200.042.6964951
GTTACCG52850.041.19139518
ACCGTTT53550.040.39137621
TGCGTTA57000.037.76261515
CGTTACC58000.037.5338217
CTGCGTT60250.035.84139614
CGTTTGA60250.035.7835423
TGTACCG60600.034.94317234
CCGTTTG62500.034.6632222
TAGCGCG17950.031.5918451
TTGGGGG241350.031.1973361
GCGTTAC72300.030.11021616
AACCCCC192500.028.4911062
TAGGGGG73750.027.054361
TCGCAAC33050.026.6903041
TTGCGAC22100.026.6097891
CCTGCGT86650.025.5675513
GCGTAAC24050.025.3220463
Produced by FastQC (version 0.11.5)