FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30521987
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG754280.24712676799187422No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA452110.14812600503368276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGC745800.028.77380270
CGCGAAC25100.028.712855
ATCGCAC809950.027.9955720
AATCGCA821750.027.6750619
TCTAGCG37750.027.6275933
TCGCACT825950.027.48340221
AAATCGC837200.027.28977418
GCGAACT37300.027.2011726
ACCACGA747900.027.04795354
ACGAACT806350.026.50689757
TTGCTCG827600.026.28320769
CGCACTG866550.026.16776822
TCCTATC846050.026.09086844
GGACTCT859600.025.6658135
GACTCTT854150.025.58055736
CGAACTG845100.025.40715258
CACGAAC804950.025.3440856
TGGACTC879550.025.18760534
CCACGAA805500.025.14409855
TTCCTAT881400.025.04387743
Produced by FastQC (version 0.11.5)