FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34757219
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG751100.21609899226977852No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530710.15269058206296654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG364010.1047293225617389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC205900.040.0485461
TACCGTT33100.034.38957620
TTACCGT34800.033.30352819
TAGCGCG15600.029.6363981
TTGGGGG297100.029.566571
ACCGTTT40050.028.42192821
TTGCGAC22250.028.3769861
GTTACCG40850.028.1177118
CGTTACC41650.027.74322317
TGCGTTA43650.025.83996215
GCGTTAC44400.025.79190816
TAGGGGG74150.025.638091
TTACCCC102750.024.7138961
CTGCGTT48200.023.90133514
AACCCCT234400.023.8559952
TTACCCG26950.023.8121931
TTGCGAG37850.023.7913931
TATCCGA51950.023.44242348
TAACCCG29200.023.3952431
TTGGGCC156100.023.251881
Produced by FastQC (version 0.11.5)