FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005420393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005420393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33336467
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG766360.22988638838062833No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG565280.16956805890678217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC175200.045.8743671
TTACCGT33050.041.19439319
TACCGTT33650.040.35616320
GTTACCG39100.034.99961518
ACCGTTT41700.032.733521
CGTTACC42050.032.54413617
TAGCGCG17950.031.7874341
TTGGGGG282250.031.2163091
TCGCAAC39600.029.524571
TAGGGGG88800.028.461421
AACCCCC198700.027.513662
TTGGGCG59750.027.4768681
AACCCCT198450.027.2132262
CTGCGTT51650.026.56247714
TTGCGAG38650.026.5369171
CGTTTGA53150.026.4057523
GCGTAAC27400.026.3133413
TTGCGAC22300.026.2146431
GCGTTAC52600.026.01678516
TGGGTAG114800.026.0099471
Produced by FastQC (version 0.11.5)