FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005421334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005421334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20960392
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG93550.024.0097878
GTATAAG56350.022.813281
GTCTCGC103000.022.647851
GTATTAG42750.022.6375981
GTCTTGC211900.020.5176321
GTGTTAC67750.020.0405771
GTGTTAG112700.019.7373331
GTATAGA37250.018.8064631
TATAAGA67250.018.4373422
TCGCTCT131300.018.209154
GTATAAC26850.017.7525731
CTGTCGC142050.017.1805069
GTATATA38200.017.0153481
GTATAAA53200.016.9691491
GTTTCAC327500.016.7234753
GTCGATA15200.016.6297661
GTATTAT40500.016.5840111
GTCTCGA247450.016.5830676
GTCTTAC76200.016.491341
TTGCTCT262550.016.4846134
Produced by FastQC (version 0.11.5)