FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005421608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005421608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13440515
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT25550.024.8363671
TCTGTCG51650.022.9961328
GTGTTAG62000.022.7961061
GTCTTGC123700.022.3850361
GTTTCAC136350.021.8627243
GGGTTTC165050.020.4905511
GTATTAG24450.020.35011
GTATTAA23650.020.1237581
TAATACT26150.020.0952764
GTATATA25150.019.7836951
GTATAAG28900.019.711771
GTCTCGC59900.019.6226251
GTGTTAC39800.019.5675741
GTTAGCC57850.019.0435163
GTACTAT20100.019.0141161
GTATTAC26300.018.3702641
TGTTAGC63050.018.2723332
GTATAGG27350.018.192321
GGTTTCA150850.018.1860662
TTGCTCT147100.017.7148974
Produced by FastQC (version 0.11.5)