FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005421609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005421609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13440515
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG75050.029.6885221
GTTAGCC73850.027.5940463
GTTTCAC159000.026.5387213
TGTTAGC78800.026.3244252
GGGTTTC186800.024.477361
GGTTTCA171900.022.4585422
GTGTTAC43050.022.0850831
GTCTCGA141400.021.591676
GTCTTGC118200.020.3855081
TTTCACC179000.020.2341384
TTAGCCA125500.019.7371524
GTATAAA32200.019.60941
CTCGATC97150.019.125088
GTGTTAA35800.018.8538841
GTCTTAC49900.018.6170271
GTCTCGC60000.018.3862551
TCTCGAT100150.018.1921067
GTGTAAC33550.018.171371
GTATTAG25850.017.9688531
TAGCCAG146050.017.8967765
Produced by FastQC (version 0.11.5)