FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39542374
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC443090.11205447604132215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG180500.018.77320724
CCTACGT191350.017.74455525
CTACGTT192250.017.69728726
TACGTTC191150.017.67363427
GACTACC205750.016.6686519
CGTACGG11550.016.6134173
ACTACCC217700.015.78521120
TACCCTA222150.015.54649622
ACCCTAC235200.014.81515923
CTCGCTA177300.014.6667637
GCGGGTT30950.014.6005011
TGACTAC244450.014.21192818
GGTTCTA240950.014.21007866
TTGACTA249050.014.03197317
TCGCTAT187050.013.9389098
CTACCCT246900.013.89075421
ATGGACC249700.013.81873342
ACGTTCT254800.013.67556628
CGCTATG192250.013.52627859
TTCTAGC285750.013.3469268
Produced by FastQC (version 0.11.5)