FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39542374
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT43050.022.2639271
GGCGATC51950.018.463282
GGTCGAC130100.016.827771
AGGTCGA123300.015.8323126
TGGCGCG174800.015.5560773
GGCGCGT210450.015.1866131
TCGCACG34050.014.48458227
CGACCTA27500.014.0722674
GTAGCGT31500.013.50556769
CGCACGA37700.013.08234328
GCGATCT82800.012.8218993
GTCGACC178700.012.4218652
CGATCTG86850.012.3027734
TCGACCC183600.012.27366353
CATTCGG39900.011.7588814
CGACCCG197600.011.6986324
GCATTCG38900.011.6210493
GGTCGTA31300.011.4884984
GTGGCGC243750.011.2992042
GCGCGTG287850.011.23422
Produced by FastQC (version 0.11.5)