FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41070463
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN518370.126214793341872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG175150.017.93085724
ACCGCGT57350.017.6156946
TACGTTC185950.016.9630227
CCTACGT184650.016.95269425
CGTACGG11200.016.8247473
CTACGTT191150.016.44793326
CCGCGTT61550.016.4136477
GACTACC198150.016.00435319
AGACCGC65500.015.5345974
CGCGTTC67200.015.0845938
ACTACCC211800.014.98959220
TACCCTA212550.014.93675522
ACCCTAC227800.014.05692223
TGACTAC236800.013.53677818
AATACGG73650.013.53317532
TTGACTA241700.013.4322817
CAGACCG79200.013.28058053
ACGTTCT242450.013.236382528
ATACGGA76250.013.20657233
CTACCCT244800.013.13686721
Produced by FastQC (version 0.11.5)