FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41982370
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC558990.13314874791489856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT60700.019.6926736
CCCTACG214350.019.67087624
CCTACGT221350.019.0801625
CCGCGTT64500.018.8510277
TACGTTC225200.018.78512627
CTACGTT228600.018.50535626
AGACCGC67850.017.7711724
GACTACC240100.017.73145119
CGCGTTC69250.017.4589738
TACCCTA250100.017.0642122
ACTACCC254900.016.87674720
TGACTAC275950.015.63874218
CTCGCTA280250.015.5456127
ACCCTAC278400.015.51434523
TTGACTA279700.015.41675217
CAGACCG80800.015.3892713
TACGGAG79250.015.21474334
CTACCCT286950.015.12326521
ATACGGA80250.015.06801533
GGTTCTA283200.015.01180366
Produced by FastQC (version 0.11.5)