FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005422198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39862622
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2154210.5404085059934091TruSeq Adapter, Index 7 (97% over 36bp)
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG1178700.29569053435571796No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT570910.1432193797989505No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC544350.13655649645926451No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC535630.13436898355557245No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC511890.12841352984758503No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT509660.1278541085430858No Hit
CTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGG509230.12774623806733035No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT508500.1275631091201176No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCC493640.12383530616726618No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC482660.12108084611192912No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC477500.11978640040286362No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG439890.11035149669783387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)