FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40875573
Sequences flagged as poor quality0
Sequence length35-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN708080.1732281526671198No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC576100.14093992027952734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG242450.017.49553724
CCTACGT255000.016.64807125
TACGTTC262600.016.20578227
CTCGCTA251700.016.1306157
CTACGTT265350.016.03765326
GACTACC273400.015.71438119
TACCCTA277250.015.49665922
TCGCTAT264200.015.40632258
CGCTATG268500.015.1595929
ACTACCC285350.015.10433220
GTCTCGC286500.014.7329595
GGTTCTA294350.014.53892966
TACGCCT20800.014.4834115
ACCCTAC299700.014.4389423
ACTACGC20400.014.0979853
ACCGCGT51950.014.0360036
TGACTAC307850.014.02256418
CTACGCC20550.013.9949954
TTGACTA311600.013.98555917
GGGGTCT331600.013.7264262
Produced by FastQC (version 0.11.5)