FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40989379
Sequences flagged as poor quality0
Sequence length35-75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1960010.4781750901861675No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT799390.19502369138112582No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG619580.15115623000777836No Hit
CCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCC539220.13155115133605708No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG532890.1300068488473563No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG507210.12374181126286399No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGT473280.11546405716466208No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC460620.1123754521872605No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTT459870.11219247795874146No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG442200.10788160513483262No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT417230.10178978315333834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC26800.031.6720053
CTACGCC29050.029.3367424
TACGCCT29850.028.8921435
CGTAGTG33100.025.97342734
CACTACG33900.025.5671422
GCGTAGT34350.024.92835433
AGTCGGT39400.021.81916632
ACCGACT41550.021.7466134
CGTACGG14650.021.2599623
AGGCGTA42250.020.8336331
ATCACCG43800.020.2565291
GAAAGCG48900.018.61729423
AGCGATC122150.018.3747563
CGATCCT123900.018.27470865
GTCGGTT34650.018.0629061
GGGGTCT495050.017.480172
CAGTCGG52300.016.76473831
ACCGCGT75100.016.7239786
TCGACCC58500.016.6897682
GTCGACC59150.016.6276441
Produced by FastQC (version 0.11.5)