FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38231387
Sequences flagged as poor quality0
Sequence length35-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT34900.018.4614941
GGCGATC36250.017.7547052
GGTCGAC51450.016.1392121
TGGCGCG108400.015.6925323
TCGCACG37350.015.67160525
CGCACGA40200.014.81590926
GGCGCGT115950.014.611824
TGCGCGA45150.014.0241479
CGTAGGG29500.013.96062764
ACGTCGG25250.013.68309766
CGCGAGT47300.013.02498511
GCGATCT58900.012.3774233
CGATCTG56750.011.8814734
GAACGTA37550.011.87247561
GTAGCGT31500.011.67845969
ACGTGCG58250.011.6344236
GGTAGTC56450.011.61684569
CCGCGCA74550.011.4777893
CGAGTCG52550.011.39811313
ACGTAGG36150.011.38855863
Produced by FastQC (version 0.11.5)