FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39302289
Sequences flagged as poor quality0
Sequence length35-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN549510.13981628398284895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA201500.015.7063387
ACCGCGT58950.015.4551656
CCCTACG126950.015.3260124
TCGCTAT211400.015.0195428
CGCTATG215100.014.7134149
CCGCGTT63400.014.3164277
TACGTTC136550.014.22369927
CCTACGT137800.014.16912325
AGACCGC65700.013.972144
CTACGTT140850.013.81371426
CGCGTTC69600.013.1888678
GACTACC149450.013.17824919
AATACGG70550.013.06153332
TACCCTA153000.012.761065522
ACTACCC157000.012.52306820
GCGGGTT31150.012.5176981
CAGACCG77600.012.4937373
GTCGACC35600.012.4069211
TTAGCGG30900.012.33458669
ATACGGG54250.012.31354733
Produced by FastQC (version 0.11.5)