FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35938232
Sequences flagged as poor quality0
Sequence length35-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC447150.12442181351603496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG176650.020.14980124
ACCGCGT44800.019.19346
CCTACGT187850.019.00316825
TACGTTC191200.018.652427
CCGCGTT46750.018.319547
CTACGTT197250.018.2192226
GACTACC206200.017.31159219
TACCCTA214050.016.86912222
ACTACCC221050.016.30361220
AGACCGC54350.016.1380484
ACCCTAC230850.015.908710523
CAGACCG59050.015.6078023
CGCGTTC57250.015.0793438
TGACTAC242300.014.91616218
TTGACTA245700.014.77940317
CTACCCT246550.014.68696921
ACGTTCT246150.014.61384128
GCTTGAC249500.014.51303415
ATGGACC249850.014.45354142
GGTTCTA249000.014.40958366
Produced by FastQC (version 0.11.5)