FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35938232
Sequences flagged as poor quality0
Sequence length35-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT39900.023.1528341
GGCGATC47850.019.7582822
GTAGCGT38350.015.68157769
TGGCGCG165700.015.4798193
GGCGCGT192950.014.3990041
GGTCGAC72600.013.86399651
CGATCTG72850.013.3034264
GCGATCT75950.013.2113283
GCATTCG32800.012.0068913
GTGGCGC219750.011.8313662
CGACCTA16400.011.4848534
TGCGACA80400.011.24588913
CGCGGTG84350.011.1154241
CGTAGGG25600.011.11320964
TCGCACG22300.011.05787625
AGGTCGA80350.011.039016
GCGCGTG254100.011.0138572
CATTCGG35300.010.9625184
GCGACAT82350.010.93795714
GGTGGCG241650.010.8837841
Produced by FastQC (version 0.11.5)