FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41229380
Sequences flagged as poor quality0
Sequence length35-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG536320.13008199492691863No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN511440.1240474632410189No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC429750.10423392250865765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG12200.019.9375743
ACCGCGT63300.016.6655866
CCCTACG213950.015.68983824
CCGCGTT67900.015.5868967
AGACCGC68550.015.4895344
AGCGATC84150.015.3011963
CCTACGT221600.015.10199325
CTCGCTA243800.014.9619037
TACGTTC225850.014.84801427
CGCGTTC70850.014.8412518
CTACGTT228150.014.68349526
GACTACC236450.014.28293619
TCGCTAT256200.014.2511558
CGCTATG261150.013.9810649
CGATCCT91150.013.77421865
TACCCTA250350.013.62739222
CGGGGTT118550.013.5920311
AATACGG78500.013.39658232
GTCGGTT30450.013.37099551
ACTACCC255550.013.32312920
Produced by FastQC (version 0.11.5)