FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39189236
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC430920.10995876520787495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC61750.028.2391856
TTCCGAT73650.023.5835275
CCGATCT85250.020.4949877
ACCGCGT71800.018.7993626
CTTCCGA94600.018.579864
CCGCGTT73600.018.3396197
AGACCGC77800.017.6155664
CCCTACG186550.017.318224
CCTACGT193350.016.76230825
TACGTTC196250.016.4624927
CTACGTT200450.016.15153126
CGCGTTC85000.016.0009068
CTCGCTA166350.015.878287
GACTACC209900.015.66887819
TCGCTAT174050.015.2939618
TACCCTA216350.015.28117422
CAGACCG93350.015.2317183
AATACGG91650.015.02870132
CGCTATG176150.015.0144079
ACTACCC221100.014.828691520
Produced by FastQC (version 0.11.5)