FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43224867
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN529760.12255908155830762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC23850.019.8128873
TACGCCT24250.019.3422055
CTACGCC25700.018.5199454
ACCGCGT69950.016.396566
CCGCGTT70550.016.1600577
CTCGCTA207700.015.8739667
AGACCGC73800.015.68261054
TCGCTAT216350.015.2709688
CGCTATG219350.015.1089379
CGCGTTC77200.014.8567468
CCCTACG147900.014.63175524
AATACGG80500.014.50884232
CAGACCG84200.014.3555443
CACTACG34950.013.8302752
TACGTTC159200.013.70133727
GAACGAT87050.013.661509545
CCTACGT159450.013.4646825
ATACGGA85850.013.36570533
CTACGTT163550.013.23200226
CGGGCTA25800.012.9499781
Produced by FastQC (version 0.11.5)