FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41485865
Sequences flagged as poor quality0
Sequence length35-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN587270.14155905873000357No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGC554210.133590079416206No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCC496160.11959736165559041No Hit
CCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCC450680.10863459156510297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT66150.021.8992426
CCGCGTT68650.021.1017447
AGACCGC74950.019.4735574
CGTACGG12200.018.5359553
CCCTACG99900.017.58798224
CAGACCG86950.017.2991923
AATACGG86550.016.7812932
TACGTTC107100.016.59765627
CCTACGT105950.016.58376725
GAACGAT88200.016.4733245
TACGGAG87700.016.44441634
ATACGGA89650.016.04834633
GTCGACC44650.016.000291
CTACGTT112100.015.76574626
AACGATC94850.015.35497546
CAATACG93750.015.30962631
GCGTTCT96600.015.0669419
CTCGCTA139100.014.9444547
TCGACCC47050.014.6376012
CGCTACG25400.014.5615827
Produced by FastQC (version 0.11.5)