FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43875722
Sequences flagged as poor quality0
Sequence length35-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC631000.14381529721607772No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT531020.12102820780932108No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN457100.1041806218026452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC24850.023.6905353
TACGCCT29450.020.4519065
CCCTACG238000.019.8601124
CTACGCC29500.019.8398554
GCGTAGT28500.019.3453233
CCTACGT246500.019.25876425
ACCGCGT40850.018.8492436
TACGTTC256900.018.49315827
CTACGTT260300.018.27746426
GACTACC267450.017.86331719
CGTAGTG30850.017.64988134
CCGCGTT44950.017.3583567
TACCCTA278150.017.213922
CTCGCTA190600.016.9493317
ACTACCC284350.016.86200320
GGTTCTA289100.016.46323266
TCGCTAT197850.016.3801698
ACCCTAC297500.016.18702723
CGCTATG202200.015.9262479
TGACTAC305950.015.78303118
Produced by FastQC (version 0.11.5)