FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36471926
Sequences flagged as poor quality0
Sequence length35-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG710870.1949088183607304No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT642080.1760477360038513No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG518400.1421367218172136No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGT506590.13889861478661697No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTT467040.1280546577112489No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC443930.12171827723054714No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT382440.10485873435913419No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG378990.10391280131463307No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC373340.10236366458958047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC49950.021.8877036
TTCCGAT56050.019.6279265
GTCGGTT29550.019.490281
CCCTACG196000.017.03139724
CCGATCT66600.016.41587
CCTACGT206900.016.18383625
ACCGCGT55350.016.0990686
GGGGTCT412850.015.9747722
TACGTTC210750.015.95330227
CTACGTT211400.015.85565726
GACTACC217700.015.52239419
TACCCTA218900.015.48452622
TTAGCGG27550.015.2595569
CCGCGTT58250.015.1799167
CGATCCT77250.015.0337865
CTTCCGA73800.015.001564
CTCGCTA409150.014.9269397
ACTACCC229500.014.82890820
AGCGATC77250.014.74373963
AGACCGC60600.014.5361964
Produced by FastQC (version 0.11.5)