FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40054200
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC473940.11832467007205237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG166650.021.73001924
ACCGCGT70750.021.3060326
CCGCGTT72900.020.58377
CCTACGT177800.020.54075825
TACGTTC180400.020.24489227
AGACCGC74950.020.0233044
CTACGTT182950.020.00009226
GACTACC193100.018.9660419
TACCCTA199400.018.31565722
CGCGTTC81750.018.313498
ACTACCC201500.018.22642520
CAGACCG90100.017.493063
AATACGG86750.017.33893232
ACCCTAC219600.016.91174723
TACGGAG91900.016.40504634
ATACGGA91950.016.39602533
GAACGAT92400.016.2809245
CTCGCTA149150.016.2624867
TGACTAC228200.016.19895618
CTACCCT228100.016.14616221
Produced by FastQC (version 0.11.5)