FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40054200
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC90100.017.4857461
GGGCGAT42950.016.4544541
TCGCACG38050.015.65929927
CGTAGGG32450.015.5328564
GGCGATC45400.015.3901012
GGCGCGT156250.015.3120121
GTAGCGT59950.015.28607369
GGTAGTC55150.014.98366669
TGGCGCG139050.014.5776773
GAACGTA36800.014.15492661
CGACCTA25800.013.934994
CGCACGA44100.013.89951828
AACGTAG38400.013.65591862
CATTCGG47400.012.9303944
GTCGACC126050.012.68940452
TCGACCC130100.012.2643983
GCATTCG48450.012.2261553
CCCCGTT91750.012.10182361
ACGTAGG43200.011.98176163
CCGCGGT107100.011.82081669
Produced by FastQC (version 0.11.5)