FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39095020
Sequences flagged as poor quality0
Sequence length35-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1108470.2835322759778611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG14700.020.7466373
ACCGCGT68900.018.4923936
AGACCGC71650.017.8856094
CCCTACG146200.017.64206324
CCGCGTT73700.017.3344787
TACGTTC155450.016.70266527
CCTACGT155550.016.58173825
CTACGTT156800.016.51514826
GACTACC164900.015.89015119
AATACGG81050.015.80859732
CAGACCG87600.015.412293
GTCGACC47850.015.3629611
ACTACCC173050.015.10266520
TACCCTA179150.014.55020222
GAACGAT88500.014.482944545
TCGACCC48850.014.4504572
ATACGGA90400.014.32535433
CGCGTTC90050.014.1871318
TACGGAG92600.014.09627334
CTCGCTA188800.013.968777
Produced by FastQC (version 0.11.5)