FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39095020
Sequences flagged as poor quality0
Sequence length35-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1099150.2811483406326432No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC512190.13101157129475827No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC496700.12704942982507747No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC423750.10838976422060918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGA202050.020.2462126
GGGCGAT41400.018.1921731
GGTCGAC233550.017.4125067
GGCGATC49500.015.2586812
GTAGCGT56750.015.15467869
TCGCACG42000.014.91731227
TGGCGCG151450.014.5701613
GCATTCG39100.014.1966543
GTCGACC296350.013.7224888
GGCGCGT191100.013.7036721
CGACCTA26900.013.5021184
GGTAGTC52200.013.480062569
TCGACCC307700.013.1721179
CGCACGA48900.013.16236328
TGCAGGT327550.012.7414073
GAACGTA37600.012.68186261
CGTAGGG34950.012.37820164
CAGGTCG341550.012.3297655
CATTCGG44550.012.3061044
AACGTAG36700.011.9673162
Produced by FastQC (version 0.11.5)