FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42532862
Sequences flagged as poor quality0
Sequence length35-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN573120.13474757471058496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG11150.018.7376253
CCCTACG179800.017.02903424
ACTACGC20000.016.953713
TACGCCT21150.016.354275
CCTACGT188250.016.26432425
TACGTTC191300.016.05957227
CTACGTT196100.015.71831826
ACCGCGT60800.015.6022656
GTCGGTT31750.015.1776451
CTCGCTA191300.015.1631217
GACTACC203300.015.14399519
TTAGCGG34300.015.05737869
TACCCTA207250.014.90560322
TCGCTAT199950.014.5756638
ACTACCC212750.014.56809520
CGCTATG203000.014.33999
AGACCGC66650.014.2342664
CCGCGTT68300.014.039697
TGACTAC226900.013.7499318
TCGACCC37200.013.4556062
Produced by FastQC (version 0.11.5)