FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38063560
Sequences flagged as poor quality0
Sequence length35-75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT41400.019.319111
GGCGCGT196200.015.7789031
GGCGATC51400.015.7268252
TGGCGCG164800.015.6880683
GTAGCGT32650.015.34032469
TCGCACG32200.015.20913127
CATTCGG46700.014.1519094
GCATTCG46150.013.9497323
GGTCGAC73500.013.5553261
CGCACGA36700.013.43765528
GCGCGTG263900.011.8761012
CGCGTGC268700.011.5717193
CGACCTA24950.011.5287794
AGGTCGA87650.011.2909736
GTGGCGC234600.011.2276922
GCGATCT74200.011.1222553
CCGCGGT92050.011.05420969
CGTAGGG28450.010.84587764
CGATCTG79550.010.7184944
GGTGGCG248550.010.6523881
Produced by FastQC (version 0.11.5)